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The study of membrane traffic in reconstituted cell-free§systems has generated an unprecedented amount of new§information on the biochemistry, molecular biology and§genetics of membrane-based molecular events that underly§normal and abnormal cellular function. Many of the§individual steps have now been isolated and dissected in§simple systems that permit detailed molecular analyses of§transport mechanisms and their regulation. Reconstituted§events of intercompartment transfer include inter-membrane§recognition and controlled membrane fusion-fission§reactions.§Provided here is an account of the current literature and§basic approaches to the investigation of the molecular§mechanisms of membrane traffic in both animal and plant§cells.Major focus is on the presentation and interpretation§of new data that provide, for the first time, a detailed§picture of the molecular events of membrane traffic and its§control.
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