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This Laboratory Manual is prepared for the course 'cryptogamic botany Practice (Biol 222)' which is given to all year twobiology students joining the biology department School of Natural and Computational Science, Hawassa University. The course has 10 laboratory studies with different sessions. Author for this manual recommend that the students should familiarize themselves by reading the experiments to be performed in the manual. Therefore, the experiments should be read before coming to the laboratory, but the practical exercise should be done in the laboratory class itself. The students should individually prepare a report and submit the report at the end of each experiment. Each lab session has three hours. The background given in each laboratory study will give sufficient theoretical information about the laboratory work